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Novel variants in LAMA3 and COL7A1 and recurrent variant in KRT5 underlying epidermolysis bullosa in
《医学前沿(英文)》 2022年 第16卷 第5期 页码 808-814 doi: 10.1007/s11684-021-0878-x
关键词: epidermolysis bullosa LAMA3 COL7A1 KRT5 Chinese families
《医学前沿(英文)》 2021年 第15卷 第3期 页码 438-447 doi: 10.1007/s11684-020-0826-1
关键词: sporadic thoracic aortic dissection exome sequencing gene COL3A1 case–control study extracellular matrix
《医学前沿(英文)》 页码 907-923 doi: 10.1007/s11684-023-1009-7
Electroactivity of the magnetotactic bacteria AMB-1 and MSR-1
《环境科学与工程前沿(英文)》 2023年 第18卷 第4期 doi: 10.1007/s11783-024-1808-3
● The first study of electrochemically active magnetotactic bacteria.
关键词: Magnetotactic bacteria Magnetospirillum magneticum Magnetospirillum gryphiswaldense Extracellular electron transfer Microbial fuel cells
<i>RR1i> and <i>RR2i> gene deletion affects the immunogenicity of a live attenuated pseudorabies
Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN
《农业科学与工程前沿(英文)》 2016年 第3卷 第1期 页码 81-86 doi: 10.15302/J-FASE-2016088
关键词: pseudorabies virus RR1 and RR2 ribonucleotide reductase vaccine candidate
Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li
《医学前沿(英文)》 2019年 第13卷 第6期 页码 646-657 doi: 10.1007/s11684-018-0643-y
关键词: androgen-independent prostate cancer normal epithelial cell-specific 1/kallikrein 10 sodium/iodide symporter radiation therapy proliferation
利用<i>cyp17a1i>缺失的伪雄鲤成功构建全雌鲤群体 Article
翟刚, 舒婷婷, 陈贶新, 娄气永, 贾景怡, 黄建飞, 石闯 , 金霞, 贺江燕, 姜冬火, 钱雪桥, 胡炜, 殷战
《工程(英文)》 2022年 第8卷 第1期 页码 181-189 doi: 10.1016/j.eng.2021.03.026
用高光谱发光法研究Cu(In<i>xi>,Ga1–<i>xi>)Se2 (CIGS)模块中由P1引起的功率损耗的成因 Article
César Omar Ramírez Quiroz, Laura-Isabelle Dion-Bertrand, Christoph J. Brabec, Joachim Müller, Kay Orgassa
《工程(英文)》 2020年 第6卷 第12期 页码 1395-1402 doi: 10.1016/j.eng.2019.12.019
关键词: Cu(Inx Ga1–x)Se2 电池到模块的效率差距 由P1引起的功率损耗 高光谱光致发光 激光烧蚀短程热效应
从H1N1血凝素序列提取的沙门氏菌传递的COBRA-HA1抗原对甲型流感亚型产生广谱保护作用 Article
Ram Prasad Aganja, Amal Senevirathne, Chandran Sivasankar, John Hwa Lee
《工程(英文)》 2024年 第32卷 第1期 页码 42-57 doi: 10.1016/j.eng.2023.08.001
A universal vaccine is in high demand to address the uncertainties of antigenic drift and the reduced effectiveness of current influenza vaccines. In this study, a strategy called computationally optimized broadly reactive antigen (COBRA) was used to generate a consensus sequence of the hemagglutinin globular head portion (HA1) of influenza virus samples collected from 1918 to 2021 to trace evolutionary changes and incorporate them into the designed constructs. Constructs carrying different HA1 regions were delivered into eukaryotic cells by Salmonella-mediated bactofection using a Semliki Forest virus RdRp-dependent eukaryotic expression system, pJHL204. Recombinant protein expression was confirmed by Western blot and immunofluorescence assays. Mice immunized with the designed constructs produced a humoral response, with a significant increase in immunoglobulin G (IgG) levels, and a cell-mediated immune response, including a 1.5-fold increase in CD4+ and CD8+ T cells. Specifically, constructs #1 and #5 increased the production of interferon-γ (IFN-γ) producing CD4+ and CD8+ T cells, skewing the response toward the T helper type 1 cell (Th1) pathway. Additionally, interleukin-4 (IL-4)-producing T cells were upregulated 4-fold. Protective efficacy was demonstrated, with up to 4-fold higher production of neutralizing antibodies and a hemagglutination inhibition titer > 40 against the selected viral strains. The designed constructs conferred a broadly protective immune response, resulting in a notable reduction in viral titer and minimal inflammation in the lungs of mice challenged with the influenza A/PR8/34, A/Brisbane/59/2007, A/California/07/2009, KBPV VR-92, and NCCP 43021 strains. This discovery revolutionizes influenza vaccine design and delivery; Salmonella-mediated COBRA-HA1 is a highly effective in vivo antigen presentation strategy. This approach can effectively combat seasonal H1N1 influenza strains and potential pandemic outbreaks.
关键词: COBRA Influenza A Salmonella Vaccine Broad spectral protection
Tian Jiang, Yuhui Hou, Tengjiang Zhang, Xudong Feng, Chun Li
《化学科学与工程前沿(英文)》 2019年 第13卷 第3期 页码 554-562 doi: 10.1007/s11705-019-1834-z
关键词: β-glucuronidase enzyme-inorganic hybrid nanoflower biotransformation glycyrrhizin glycyrrtinic acid 3-O-mono-β-D-glucuronide
131I标记的人源化抗B7-H3抗体用于胶质母细胞瘤放射免疫疗法的治疗特性 Article
傅丰庆, Meng Zheng, Shandong Zhao, Yan Wang, Minzhou Huang, Hanqing Chen, Ziyi Huang, Kaijie Zhang, 缪丽燕, 张学光
《工程(英文)》 2023年 第30卷 第11期 页码 190-202 doi: 10.1016/j.eng.2023.05.011
B7 homolog 3 (B7-H3) has attracted much attention in glioblastoma (GBM) radioimmunotherapy (RIT) due to its abnormally high expression on tumor cells. In this study, we report that two specific humanized anti-human B7-H3 antibodies (hu4G4 and hu4H12) derived from mouse anti-human B7-H3 antibodies that were generated by computer-aided design and exclusively recognize membrane expression of B7-H3 by human glioma cells. Hu4G4 and hu4H12 were radiolabeled with 89Zr for RIT antibody screening. Micro-positron emission tomography (PET) imaging, biodistribution and pharmacokinetic (PK) analyses of 89Zr-labeled antibodies were performed in U87-xenografted models. 125I labelling of the antibodies for single-photon emission computed tomography (SPECT) imaging was also used to investigate the biological behavior of the antibodies in vivo. Furthermore, the pharmacodynamic (PD) of the 131Ilabeled antibodies were evaluated in U87-xenografted mice and GL261 Red-FLuc-B7-H3 in situ glioma tumor models. Micro-PET imaging and biodistribution analysis with a gamma counter showed that 89Zr-deferoxamine (DFO)-hu4G4 had higher tumor targeting performance with lower liver uptake than 89Zr-DFO (hu4H12, immunoglobulin G (IgG)). The biodistribution results of 125I-SPECT imaging were similar to those of 89Zr-PET imaging, though the biodistribution in long bone joints and the thyroid varied. The PD analysis results indicated that 131I-hu4G4 had an excellent therapeutic effect and high safety with no apparent toxicity. Interestingly, 131I-hu4G4 improved the tumor vasculature in tissues with higher expression of collagen type IV and platelet-derived growth factor receptor b (PDGFR-b) compared with control treatment, as determined by immunofluorescence (IF), which contributed to inhibiting tumor growth. Taken together, our data indicate that hu4G4 exhibits good tumor targeting and specificity, achieves low nonspecific concentrations in normal tissues, and has acceptable PK characteristics. 131I-hu4G4 also exerts effective antitumor effects with an ideal safety profile. Therefore, we expect hu4G4 to be an excellent antibody for the development of GBM RIT.
关键词: B7-H3 Radioimmunotherapy Glioblastoma Pharmacokinetics Pharmacodynamics
Qiao ZHANG,Yu HONG
《环境科学与工程前沿(英文)》 2014年 第8卷 第5期 页码 703-709 doi: 10.1007/s11783-014-0649-x
关键词: Scenedesmus sp. LX1 Chlorella sp. HQ growth rate algal biomass lipid accumulation triacylglycerols (TAGs)
Genome-wide search for candidate genes determining vertebrae number in pigs
Longchao ZHANG, Jingwei YUE, Xin LIU, Jing LIANG, Kebin ZHAO, Hua YAN, Na LI, Lei PU, Yuebo ZHANG, Huibi SHI, Ligang WANG, Lixian WANG
《农业科学与工程前沿(英文)》 2017年 第4卷 第3期 页码 327-334 doi: 10.15302/J-FASE-2017163
关键词: genome-wide association study number of vertebrae pig SSC7 TGFβ3
《医学前沿(英文)》 2023年 第17卷 第4期 页码 729-746 doi: 10.1007/s11684-022-0978-2
关键词: hepatocellular carcinoma YAP1 Akkermansia muciniphila anti-PD-1 dihydroartemisinin bile acid
磁控溅射法生长的带隙可调谐(GaxIn1−x)2O3 Research Articles
张法碧1,孙巾寓1,李海鸥1,周娟1,王荣1,孙堂友1,傅涛1,肖功利1,李琦1,刘兴鹏1,张秀云1,郭道友2,王相虎3,秦祖军1
《信息与电子工程前沿(英文)》 2021年 第22卷 第10期 页码 1370-1378 doi: 10.1631/FITEE.2000330
标题 作者 时间 类型 操作
Novel variants in LAMA3 and COL7A1 and recurrent variant in KRT5 underlying epidermolysis bullosa in
期刊论文
Identification of variants associated with sporadic thoracic aortic dissection: a case--control study
期刊论文
High frequency of alternative splicing variants of the oncogene in neuroendocrine tumors of the pancreas and breast
期刊论文
<i>RR1i> and <i>RR2i> gene deletion affects the immunogenicity of a live attenuated pseudorabies
Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN
期刊论文
<i>NES1i>/<i>KLK10i> and <i>hNISi> gene therapy enhanced iodine-131 internal radiation in PC3 proliferation
Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li
期刊论文
用高光谱发光法研究Cu(In<i>xi>,Ga1–<i>xi>)Se2 (CIGS)模块中由P1引起的功率损耗的成因
César Omar Ramírez Quiroz, Laura-Isabelle Dion-Bertrand, Christoph J. Brabec, Joachim Müller, Kay Orgassa
期刊论文
从H1N1血凝素序列提取的沙门氏菌传递的COBRA-HA1抗原对甲型流感亚型产生广谱保护作用
Ram Prasad Aganja, Amal Senevirathne, Chandran Sivasankar, John Hwa Lee
期刊论文
Construction of a CaHPO4-PGUS1 hybrid nanoflower through protein-inorganic self-assembly, and its application
Tian Jiang, Yuhui Hou, Tengjiang Zhang, Xudong Feng, Chun Li
期刊论文
131I标记的人源化抗B7-H3抗体用于胶质母细胞瘤放射免疫疗法的治疗特性
傅丰庆, Meng Zheng, Shandong Zhao, Yan Wang, Minzhou Huang, Hanqing Chen, Ziyi Huang, Kaijie Zhang, 缪丽燕, 张学光
期刊论文
Comparison of growth and lipid accumulation properties of two oleaginous microalgae under different nutrient conditions
Qiao ZHANG,Yu HONG
期刊论文
Genome-wide search for candidate genes determining vertebrae number in pigs
Longchao ZHANG, Jingwei YUE, Xin LIU, Jing LIANG, Kebin ZHAO, Hua YAN, Na LI, Lei PU, Yuebo ZHANG, Huibi SHI, Ligang WANG, Lixian WANG
期刊论文
Dihydroartemisinin increased the abundance of by YAP1 depression that sensitizes hepatocellular carcinomato anti-PD-1 immunotherapy
期刊论文